Vol. 7, Issue 7, Part H (2025)

Canine Transmissible Venereal Tumor (CTVT) is a naturally occurring contagious neoplasm that primarily affects sexually active dogs. Chemotherapy with vincristine sulfate remains the treatment of choice with high success rates. This study evaluated the use of vaginal cytology as a simple, non-invasive tool to monitor the therapeutic response of five bitches diagnosed with CTVT undergoing vincristine sulfate therapy. Vaginal smears were collected before and after each weekly dose and evaluated microscopically. A progressive decline in neoplastic cell population with a corresponding increase in superficial epithelial cells was observed, correlating with clinical tumor regression. Vaginal cytology may serve as a valuable adjunct to clinical evaluation during therapy.