Pinaki Raj, Sumita Dasgupta, AabhaBhave
Endophytes are microorganisms that colonize within the living plant cell and mostly have a symbiotic association with the host plant. There is a growing interest to recognize the involvement of the endophytes in the production of the growth promoting bioactive compounds and secondary metabolites that are produced by the plants. Medicinal plants are known to produce several phytochemicals with potential biological activities. In the current study the endophytic bacterial diversity of two valuable medicinal plants Gymnema sylvestre, and Tabernamontana divaricate are studied. The study aimed at understanding the involvement of the endophytic bacterial community in growth beneficial trait of the host plant viz. IAA( Indole Acetic Acid) production, Phosphate solubilisation and their capacity to grow on Nitrogen free medium so as to assert their role in Nitrogen fixation. A total of 14 bacterial strains were isolated from both the taxa. 11 isolates from Gymnema sylvestre and 03 from Tabernamontana divaricate. The morphological and biochemical characterization of the isolates were performed. It was found that 13 out of 14 isolates were capable of producing IAA, 04 isolates were able to grow on Nitrogen free media and only one was capable of Phosphate solubilisation. The study showed that the isolated strains of endophytes have immense plant growth promoting potential which can be used for improvement of growth of crops.
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