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How to Write the Materials and Methods Section of an Agriculture Paper

How to Write the Materials and Methods Section of an Agriculture Paper

The Materials and Methods section must let a competent reader repeat your work. For agricultural research that means the study area and its soil, the season and year, the crop or organism with its variety or strain, the design, the replications, the treatments, the measurements and the statistical analysis — written in the past tense, in the order the work was done.

This is where most agriculture journal papers are actually lost. Reviewers rarely reject a paper because the result is wrong; they reject it because they cannot tell what was done well enough to judge whether it is right.

The test to apply to every sentence

Could a competent researcher in my field, with access to a similar site and similar equipment, repeat this experiment from what I have written?

For any agriculture journal, that is the only question. Every sentence either serves it or does not, and the ones that do not can go.

What has to be in there

ElementWhat to stateCommon failure
Study areaLocation, latitude and longitude, elevation, agro-climatic zone“A field experiment was conducted” — where?
Season and yearKharif or rabi, and the yearsOmitted, so the result cannot be placed in time
SoilTexture, pH, organic carbon, available NPK, classificationAssumed to be obvious
WeatherRainfall and temperature over the growing periodLeft out, though it often explains the result
MaterialCrop and variety, or strain, isolate, breed, with source“Wheat was sown” — which variety?
DesignRBD, split plot, factorial — name it, with plot size“Experiments were conducted”
ReplicationsHow manyThe most common omission of all
TreatmentsWhat was applied, at what rate, whenRates given without timing
MeasurementsWhat was recorded, how, at what stageMethod of measurement unstated
AnalysisTest, significance level, software and versionMeans reported with no test

Write it in the order the work happened

An agriculture journal Methods section following the actual sequence — site, soil, design, treatments, crop management, measurements, analysis — is easier to follow and easier to check. A section organised by theme forces the reader to reconstruct the order, and reviewers who have to reconstruct anything tend to ask for clarification.

Use subheadings where the work has distinct stages. Four short labelled blocks read far better than one long paragraph covering all four.

A before and after

Here is a Methods paragraph as it usually arrives, and the same paragraph after the missing information is added. Nothing was re-tested between the two.

As submitted

A field experiment was conducted to study the effect of different nitrogen levels on wheat. The crop was raised with recommended practices and data were analysed statistically.

After revision

A field experiment was conducted during the rabi seasons of 2024–25 and 2025–26 at the research farm, Ludhiana, Punjab (30°54′N, 75°48′E, 247 m above mean sea level), in a sandy loam soil (pH 7.8, organic carbon 0.42%, available N 210 kg ha-1). Wheat variety HD 3086 was sown at 100 kg ha-1 in the first week of November in plots of 5 × 4 m. The experiment was laid out in a randomised block design with four replications and three nitrogen schedules: a single basal dose, a two-way split at sowing and first irrigation, and a three-way split at sowing, first irrigation and boot stage, all at 120 kg N ha-1. Grain yield, thousand-grain weight and apparent nitrogen recovery were recorded at harvest. Data were analysed by one-way ANOVA at a 5% significance level using [software, version].

The second version answers every question a reviewer would raise, and it took about twenty minutes because none of it required new work — it was all in the field record.

Standards and published methods: cite and specify

In an agriculture journal paper, citing an established method saves a page and is the right thing to do. But most methods permit a range of choices, and the reviewer needs to know which you made — sample size, extraction ratio, incubation temperature, the instrument. If you deviated in any way, say so and say why. An unexplained deviation discovered later is far worse than one declared.

Laboratory, glasshouse and survey studies

The principle is the same but the details differ, and each type is rejected for thin methods just as often as field trials.

Laboratory and analytical work

State the sample size and how samples were drawn, the extraction or digestion procedure with reagent concentrations, the instrument with its make and model, the calibration standards, and the number of analytical replicates. “Nitrogen was estimated by the Kjeldahl method” is a citation, not a method.

Glasshouse and pot experiments

Pot size, growing medium and its composition, number of plants per pot, light and temperature regime, watering schedule, and the number of pots per treatment. Pot studies are frequently reported more thinly than field trials, and reviewers notice.

Surveys and socio-economic studies

Sampling frame, sampling method, sample size and how it was arrived at, the period of data collection, the instrument used, and how non-response was handled. A survey with an unstated sampling method cannot be evaluated at all.

Weather data: the section people skip

For any field study in an agriculture journal, weather over the growing period is not optional context — it is often the explanation for the result. A treatment that worked in a year with well-distributed rainfall and failed in a dry one has told you something, and a reader cannot see that if the rainfall is missing.

Give monthly rainfall and mean maximum and minimum temperature over the crop period, and say where the data came from. Two sentences or a small table. If your result differs between seasons, this is usually where the answer is.

Numbers that must appear

  • How many. Replications, plants sampled, pots, respondents, analytical repeats.
  • How much. Rates with units and a space — 120 kg N ha-1, not 120kg.
  • When. Sowing date, application timing, sampling stage.
  • Where. Coordinates, elevation, agro-climatic zone.
  • What software. Name and version. “Data were analysed using standard statistical software” is not repeatable.

What to leave out

  • Results. They belong in Results, even ones that emerged during method development.
  • Justification. Why you chose an approach belongs in the Introduction or Discussion.
  • Textbook explanation. Your readers know what a randomised block design is. Name it and move on.
  • Irrelevant conditions. Detail that cannot affect the result is noise.

The check that catches everything

Before it goes to the agriculture journal, give your Materials and Methods to a colleague who did not do the work and ask them to describe the experiment back to you. Not to comment on it — to describe it.

Every point where their description differs from what you actually did is a sentence to rewrite. Fifteen minutes, and it finds the ambiguities you cannot see because you know what you meant and they only know what you wrote. It is the most effective review any paper gets before submission.

Formatting requirements — units, symbols, italics for species names — are on the Instructions to Author page, and our pre-submission checklist covers the rest.

Frequently Asked Questions

What must the Materials and Methods section contain?

Enough for someone else to repeat the work: the study area, the season and year, the material with its variety or strain, the experimental design, the number of replications, the treatments, the measurements taken, and the statistical analysis with software.

What tense should Materials and Methods be in?

Past tense, because you are describing what was done.

Do I need to give the soil type?

Yes, where it can affect the result — which in most field research it can. Texture, pH and organic carbon at minimum, and the classification if you have it.

Can I just cite a standard method instead of describing it?

Cite it and state anything specific to your use — sample size, dilution, incubation time, any deviation. A citation alone leaves a reviewer unable to judge what you actually did.

How much detail is too much?

If removing a detail would stop someone repeating the work, keep it. If it would not, cut it.

What is the single most common omission?

The number of replications. It is one sentence and it generates more reviewer comments than any other missing item.

Related Guides

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Created by Kwame Asante  •  09 Aug 2026